This third European Seminar on Accelerator Alignment will be held at the ESRF from 3 - 5 June 2025, following the success of the previous seminars in June 2023 and April 2024 at the ESRF and CERN. This seminar serves as a platform for knowledge exchange and collaboration, fostering a strong European community dedicated to Accelerator, Detector, and Beamline Alignment. Discussions will cover a range of key topics, including advanced instruments, measurement techniques, uncertainty analysis, the Engineering to Alignment (E2A) workflow, software solutions, artificial intelligence, machine learning, and workforce development challenges.